Genome encapsidation by orthobunyavirus nucleoproteins

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Genome encapsidation by orthobunyavirus nucleoproteins.

All negative-sense, single-stranded RNA (−ssRNA) viruses encode a nucleoprotein (NP), the major function of which is to bind viral RNAs and encapsidate them as ribonucleoprotein complexes (RNPs) (1, 2). Consequently, the genomes of −ssRNA viruses do not exist as naked RNA but rather as protein–RNA complexes with high-order structures. In PNAS, Li et al. (3), Niu et al. (4), and Reguera et al. (...

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Structure of Schmallenberg orthobunyavirus nucleoprotein suggests a novel mechanism of genome encapsidation.

Schmallenberg virus (SBV), a newly emerged orthobunyavirus (family Bunyaviridae), has spread rapidly across Europe and has caused congenital abnormalities in the offspring of cattle, sheep, and goats. Like other orthobunyaviruses, SBV contains a tripartite negative-sense RNA genome that encodes four structural and two nonstructural proteins. The nucleoprotein (N) encapsidates the three viral ge...

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Structure of the Leanyer orthobunyavirus nucleoprotein-RNA complex reveals unique architecture for RNA encapsidation.

Negative-stranded RNA viruses cover their genome with nucleoprotein (N) to protect it from the human innate immune system. Abrogation of the function of N offers a unique opportunity to combat the spread of the viruses. Here, we describe a unique fold of N from Leanyer virus (LEAV, Orthobunyavirus genus, Bunyaviridae family) in complex with single-stranded RNA refined to 2.78 Å resolution as we...

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Genetic elements regulating packaging of the Bunyamwera orthobunyavirus genome.

The genome of Bunyamwera virus (BUN; family Bunyaviridae, genus Orthobunyavirus) comprises three segments of negative-sense, single-stranded RNA. The RNA segments are encapsidated by the viral nucleocapsid (N) protein and form panhandle-like structures through interaction of complementary sequences at their 5' and 3' termini. Transcription and replication of a BUN genome analogue (minireplicon)...

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Etymologia: Orthobunyavirus

21. Grimstad PR, Shabino CL, Calisher CH, Waldman RJ. A case of encephalitis in a human associated with a serologic rise to Jamestown Canyon virus. Am J Trop Med Hyg. 1982; 31:1238–44. 22. Centers for Disease Control and Prevention. Human Jamestown Canyon virus infection—Montana, 2009. MMWR Morb Mortal Wkly Rep. 2011;60:652–5. 23. Artsob H. Arbovirus activity in Canada. Arch Virol. 1990; Suppl:...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 2013

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.1306838110